A simple, swab-based diagnostic test for the detection of Listeria spp. on food contact surfaces. Ideal for ensuring regulatory compliance and minimising the risk of Listeria contamination.
Highly sensitive, easy-to-use, and impressively quick, the test produces a distinct black/brown colour change for positive results.
Intended Use
The Listeria Isolation Swab is designed to be used alongside traditional selective methods to minimise the risk of Listeria contamination. This easy-to-use diagnostic test can be applied anywhere in the environment where the presence of Listeria spp. would be critical.
Listeria spp. and specifically Listeria monocytogenes, are rapidly becoming the most important pathogen in the Food Industry. Regulatory bodies worldwide insist that all food products are Listeria-free.
The Listeria Isolation Swab works on an enhanced esculin media formulation. The hydrolysis of esculin gives a distinctive black/brown precipitate. Inhibitors in the media suppress the growth of non-Listeria spp., allowing only Listeria spp. to grow and produce the characteristic black/brown precipitate.
Why choose Klipspringer Environmental Swabbing Tests?
- Highly sensitive, so will detect even low levels of the target organism
- Organisms remain viable for further testing
- Easy to use, with highly visible results
- Fast results, helping to speed up the process of taking corrective action
Instructions for Use
- Peel back wrapper to expose both caps.
- Remove swab and sample test site by rubbing swab over as large an area as practicable.
- Remove cap of culture tube with thumb and forefinger and discard.
- Insert swab in culture tube and push down fully to immerse the swab completely.
- Fill in time, date, and site details.
- Incubate at 37°C for up to 48 hours.
- Read results. A positive result is indicated by a colour change from Light Brown Agar to Black/Dark Brown commencing around the bud. Any colour change is significant. A negative result shows no colour change.
- Record results and dispose of tube.
Precautions
- If any black media tubes are found when the box is opened, DO NOT USE.
Precautions When Swabbing
- When sampling an area always use the same swabbing technique.
- Always try to sample the same surface are (100 cm² or 200 cm²), and be consistent in your sampling.
- The moisture level on the surface should not vary at different sample times. It may be preferable with dry surfaces to wet the swab in a suitable wetting agent such as Ringers, Tween Lecithin Thiosulphate Ringers or Tween Peptone water.
Example Of Area To Be Swabbed

Visual Result

Storage
Store the tests at 5-25°C. Do not freeze.
Shelf Life
- 12 months from the date of manufacture.
- The expiration date is printed on the tube label, peel pouch, and box label.
Pack Size | 125 Swabs |
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